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CD73分子(CD73)多克隆抗體

Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

  • CD73分子(CD73)多克隆抗體產(chǎn)品包裝(模擬)
  • CD73分子(CD73)多克隆抗體產(chǎn)品包裝(模擬)
  • PAB250Bo01.jpgFigure. Western Blot; Sample: Recombinant NT5E, Bovine.
  • CD73分子(CD73)多克隆抗體Western Blot; Sample: Bovine Cerebrum lysate;
    Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗體Knockout Varification:
    Lane 1: Wild-type K562 cell lysate;
    Lane 2: NT5E knockout K562 cell lysate;
    Predicted MW: 63kd
    Observed MW: 70kd
    Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗體Western Blot; Sample: Bovine Liver lysate;
    Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗體Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 3μg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對CD73的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CD73。

用法

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào)適用物種:Bos taurus; Bovine (Cattle,牛)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB250Bo01CD73分子(CD73)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Bo01CD73分子(CD73)多克隆抗體WB; IHC; ICC; IP.

參考文獻(xiàn)

雜志參考文獻(xiàn)
Journal of AutoimmunityThe expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation[Pubmed:25640206]
TalantaSimultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?…[10.1016:j.talanta.2018.01.044]
Cancer?BiomarkersExpression and clinical significance of serum NT5E protein in patients with colorectal cancer[Pubmed: 30932882]