YY肽(PYY)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Peptide YY (PYY)
PYY1; Peptide tyrosine tyrosine
- 編號KSB067Hu11
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.77pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對YY肽(PYY)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB067Hu01 | YY肽(PYY)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SPB067Hu01 | YY肽(PYY)合成多肽 | Immunogen; Blocking Peptide. |
| CPB067Hu21 | YY肽(PYY)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAB067Hu02 | YY肽(PYY)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB067Hu01 | YY肽(PYY)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB067Hu22 | YY肽(PYY)單克隆抗體 | WB; IHC; ICC; IP. |
| CEB067Hu | YY肽(PYY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB067Hu | YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB067Hu11 | YY肽(PYY)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Sport Studies | The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men[Com:Source] |
| Annals of Applied Sport Science | Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes[Aassjournal:Source] |
| Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents.[pubmed:28413639] |
| Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes[pubmed:28620678] |
| Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents[10.3892/br.2017.865] |
| Journal of Diabetes & Metabolism | High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors[10.4172/2155-6156.1000733?] |
| Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21[Pubmed:29593555] |
| Food?and Nutrition?Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet[Pubmed:29599686] |
| The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs[Pubmed: 31175811] |
| The Journal of Nutritional Biochemistry | Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity[Pubmed: 31812910] |
| Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo[34148100] |
| foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with?…[34828792] |