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中腦星形膠質(zhì)細(xì)胞來源神經(jīng)營養(yǎng)因子(MANF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

  • 中腦星形膠質(zhì)細(xì)胞來源神經(jīng)營養(yǎng)因子(MANF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 中腦星形膠質(zhì)細(xì)胞來源神經(jīng)營養(yǎng)因子(MANF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)中腦星形膠質(zhì)細(xì)胞來源神經(jīng)營養(yǎng)因子(MANF)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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參考文獻(xiàn)

雜志參考文獻(xiàn)
PLoS One.Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing[PubMed: PMC3378547]
Endocr J.Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.[pubmed:28216543]
Journal of Steroid Biochemistry and Molecular BiologyDepressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling[pubmed:28263910]
Endocrine JournalCirculating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance[10.1507:endocrj.EJ16-0472]
Nature?MetabolismMANF regulates metabolic and immune homeostasis in ageing and protects against liver damage[]
J Exp MedFeeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway[33856409]
J Inflamm ResThe Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia?…[34234505]