乳腺絲抑蛋白(Maspin)多克隆抗體
Polyclonal Antibody to Mammary Serine Protease Inhibitor (Maspin)
SERPINB5; PI5; Protease Inhibitor 5; Serpin Peptidase Inhibitor Clade B Member 5
- 編號PAD386Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPD386Hu01-乳腺絲抑蛋白(Maspin)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種-
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human Maspin Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
WB圖
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human Maspin Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對Maspin的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別Maspin。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學(xué)：5-20μg/mL;1:25-100
免疫細胞化學(xué)：5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD386Hu01 | 乳腺絲抑蛋白(Maspin)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD386Hu01 | 乳腺絲抑蛋白(Maspin)多克隆抗體 | WB; IHC |
| FAD386Hu01 | 抗乳腺絲抑蛋白(Maspin)多克隆抗體 | FCM |
| MAD386Hu21 | 乳腺絲抑蛋白(Maspin)單克隆抗體 | WB; IHC; ICC; IP. |
| SED386Hu | 乳腺絲抑蛋白(Maspin)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD386Hu | 乳腺絲抑蛋白(Maspin)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| International Journal of Cancer | Serpin B5 is a CEA-interacting biomarker for colorectal cancer[Onlinelibrary: ijc.28494] |
| Molecular and Cellular Biochemistry | Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway[Pubmed:29363056] |