Bone Morphogenetic Protein 8B (BMP8B) is a protein that belongs to the bone morphogenetic protein family, which is involved in the regulation of growth and differentiation of various cell types, including osteoblasts, chondrocytes, and neurons. It plays a role in skeletal development and tissue repair processes.Besides,Bone Morphogenetic Protein 8A (BMP8A) has been identified as an interactor of BMP8B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse BMP8B and recombinant human BMP8A. Briefly, a functional ELISA assay was conducted to detect the interaction of recombinant mouse BMP8B and recombinant human BMP8A. Briefly, BMP8B was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP8A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BMP8B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse BMP8B and recombinant human BMP8A was shown in Figure 1, the EC50?for this effect is 0.078ug/mL.