27kDa熱休克蛋白(Hsp27)重組蛋白
Recombinant Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- 編號(hào)RPA693Bo01
- 物種Bos taurus; Bovine (Cattle，牛)相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞質(zhì)
- 預(yù)測(cè)分子量26.1kDa
- 實(shí)際分子量26kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Met1~Lys201 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.0
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
SDS-PAGE圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA693Bo01 | 27kDa熱休克蛋白(Hsp27)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA693Bo01 | 27kDa熱休克蛋白(Hsp27)多克隆抗體 | WB; ICC/IF |
| MAA693Bo21 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA693Bo | 27kDa熱休克蛋白(Hsp27)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA693Bo | 27kDa熱休克蛋白(Hsp27)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA693Bo01 | 27kDa熱休克蛋白(Hsp27)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGA693Bo01 | 27kDa熱休克蛋白(Hsp27)引物對(duì) | PCR |
| CGA693Bo01 | 27kDa熱休克蛋白(Hsp27)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells[PubMed: 26467738] |
| Nature?Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis[Pubmed:29650953] |
| Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028] |
| Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity[Pubmed: 31102684] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort[Pubmed: 32210109] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771] |
| Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation[] |